General Stability | Organism |
---|---|
high concentrations of glycerol are essential in preventing deactivation of the enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the enzyme kinetics are not exactly conform with Michaelis-Menten kinetics, the curve is hyperbolic or the activity of lignoceroyl-CoA : sphingosine lignoceroyltransferase in the liver preparation increases in a somewhat sigmoidal fashion with increasing lignoceroyl-CoA concentration up to about 0.004 mM | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingosine + lignoceroyl-CoA | Rattus norvegicus | - |
N-lignoceroylsphingosine + CoA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 2.7 and 4.9fold from liver and brain microsomes partially, respectively, by gel filtration | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-20°C, several months | Rattus norvegicus |
4°C, 48 h, liver enzyme loses 70% of activity, brain enzyme looes 41% of activity | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sphingosine + lignoceroyl-CoA | - |
Rattus norvegicus | N-lignoceroylsphingosine + CoA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ceramide synthetase | - |
Rattus norvegicus |
lignoceroyl-CoA : sphingosine lignoceroyltransferase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |